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. 2014 Sep 25;10(9):e1004380. doi: 10.1371/journal.ppat.1004380

Figure 3. Proliferative capacity virus-specific CD8 T-cell populations is influenced by co-inhibitory molecule expression.

Figure 3

CD8 T-cell populations were sorted on the basis of 2B4, PD-1 and CD160 expression, labeled with CFSE and stimulated with (viral) peptides in the presence of autologous irradiated CD8-depleted PBMCs (ratio CD8/feeder cells 1∶10) for 6 days (n = 11). (A) Representative CMV (#1260 CMV HLA-A*01 VTEHDTLLY) and CMV (#1261 CMV HLA-A*02 NLVPMVATV) specific CD8 T-cell proliferation capacity assessed by CFSE based assay. (B) Proliferation index (CFSElow CD8 T-cell frequency/Multimer-specific CD8 T-cell frequency) of virus-specific CD8 T cells. Red bars correspond to mean ± SEM. Red stars indicate statistical significance (P<0.05). Statistical significance (P values) in panel B was obtained using One-way ANOVA (Kruskal-Wallis test) followed by a Student's t-test.