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. 2014 Sep 25;10(9):e1004380. doi: 10.1371/journal.ppat.1004380

Figure 4. Intrinsic proliferation capacity of sorted CD8 T-cell populations expressing the different combinations of 2B4, CD160 and PD-1.

Figure 4

CD8 T-cell populations were sorted on the basis of 2B4, PD-1 and CD160 expression, labeled with CFSE and stimulated in anti-CD3 and anti-CD28 MAbs coated plate for 6 days (n = 11). The percentage of proliferating CD8 T cells (CFSE low) was assessed by flow cytometry at day 6. (A) Representative flow cytometric profiles of proliferating CD8 T-cell populations following anti-CD3/CD8 MAbs stimulation. Unstimulated cells (negative control) are also shown. (B) Percentage of CD8 T-cell proliferation of each CD8 T-cell subset upon 6 days stimulation with a-CD3/CD28 coated mAbs. Red bars correspond to mean ± SEM. Red stars indicate statistical significance (P<0.05). NS: not significant. Statistical significance (P values) in panels A and B were obtained using One-way ANOVA (Kruskal-Wallis test) followed by a paired Student's t-test.