Table 1. Primer sequences used for cDNA cloning, real-time quantitative PCR and dsRNA synthesis.

Gene	Primer sequence (5′→3′)	Fragment length (bp)
PCR	CCACCATTCAACCTATCAC	759
	AACATCGTCTTTGCCTTC
3′RACE-GSP1	ACACCGAGCGTGGACAAAGAGGA	1178
3′RACE-GSP2	CTATTGCCGCCAAGAAAGCATCCAG
5′RACE-GSP1	TTGGCGGCAATAGCGTTCCAGTCC	2509
5′RACE-GSP2	CTCTTTGTCCACGCTCGGTGTCTT
Real-time quantitative PCR
NADH-F	ATAGTTGGCTGTAGAACCAGAGTG	96
NADH-R	ACACGAAGGGAAGAGCACATA
BtTRP-F	GAAGACACCGAGCGTGGACAAAG	217
BtTRP-R	GGCAATAGCGTTCCAGTCCTTTT
dsRNA synthesis primers
	TAATACGACTCACTATAGGGAGACCAC GAAGACACCGAGCGTGGACAAAG	244
	TAATACGACTCACTATAGGGAGACCAC GGCAATAGCGTTCCAGTCCTTTT

Primer sequences (no underline) are shown for PCR amplification, rapid amplification of cDNA ends (RACE) of BtTRP gene, and the relative quantification real-time PCR for detecting BtTRP mRNA expression patterns; primer sequences plus T7 promoter sequences (underlined) are shown for production of dsRNA transcription templates.