Figure 2. Scara5 was found both in the nucleus and cytoplasm of retinal cells.

A: Scara5 expression was observed at nuclear (arrow) and cytoplasmic (arrowhead) compartments in mouse retinal histological sections immunolabeled with a specific anti-Scara5 antibody. Nuclei were marked with Hoechst Stain Solution. B: Western blotting analysis of nuclear and cytoplasmic protein fractions samples confirmed the nuclear and cytoplasmic Scara5 content in the retinal cells. α-tubulin was used as a loading control. Topoisomerase-I (Topo I) was used to assure that nuclear protein was not present in the cytoplasmic fraction. GL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar: 28 µm.