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. 2014 Sep 26;9(9):e107213. doi: 10.1371/journal.pone.0107213

Figure 5. Modulation of regulatory T cell (Tregs) populations.

Figure 5

(A) DM199 increases Tregs in pancreatic lymph nodes (PLN). Single cell suspensions of PLN were stained for CD4, CD25, CD127 and Foxp3, and analyzed by FACS. The percentage of CD25+Foxp3+CD127− Tregs within the total CD4+ T cells population was calculated. Data are mean ± SEM (n = 4–10 mice/group). (B) Upper panels: representative images of pancreatic islets stained with anti-Foxp3 (red) and anti-CD4 (green) antibodies, from mice treated for 10 weeks with either vehicle or 100 U/kg/day of DM199. Lower panels: representative images of islets immuno-stained for insulin (brown) and counterstained with H&E. (C) The proportion of CD4+Foxp3+ Tregs in pancreatic islet infiltrates was calculated for each of at least 40 islets per animal. Data are presented as mean ± SEM (n = 8–9 mice/group). *P<0.05 vs. control, ***P<0.001 vs. control, using one-way ANOVA with Tukey post-hoc multiple comparisons test.