Table 3. Activities of key glucose catabolic enzymes in P. fluorescens strains wild type (WT), plasmid transformants harbouring yc operon, Int (genomic integrants of yc operon) grown in TRP broth (pH 8.0) minimal media.
| P. fluorescens strains | Enzyme Activitya | |||||
| CS | G6PDH | ICDH | PYC | ICL | GDH | |
| PfO-1WT | 66.87±5.8 | 234.42±4.42 | 233.56±12.4 | 7.48±0.72 | 3.11±0.44 | 34.02±7.36 |
| PfO-1 Pyc | 177.1±18.3††† | 118.22±5.08††† | 204.99±7.84 | 38.26±2.93††† | 1.35±0.22 | 54.41±5.43 |
| PfO-1Int | 212.9±16††† | 114.23±7.35††† | 224.16±9.38 | 37.13±3.78††† | 5.28±0.93 | 76.30±8.55†††,‡ |
| Pf5-1WT | 37.2±3.66 | 223.88±20.4 | 140.66±11.75 | 16.72±2.78 | 1.5±0.17 | 54.65±7.76 |
| Pf5-1 pYC | 171.99±7.12††† | 19.18±3.53††† | 239.96±25.75††,‡ | 12.01±2.55 | 1.27±0.16 | 91.33±4.4†† |
| Pf5-1Int | 175.15±8.34††† | 25.77±5.16††† | 162.56±28.3 | 11.74±1.57 | 4.01±0.56†††,‡‡‡ | 103.20±9.09††,‡‡‡ |
| CHAO-1WT | 38.1±3.98 | 41.56±4.17 | 261.36±13.07 | 10.13±2.42 | 2.36±0.16 | 27.10±4.89 |
| CHAO-1 pYC | 103.2±8.53††† | 24.67±4.85†† | 278.25±11.5 | 47.20±14†† | 2.45±0.22 | 23.54±3.68 |
| CHAO-1Int | 158.5±16.1†††,‡‡ | 123.59±6.65†††,‡‡‡ | 261.98±25.45 | 32.53±5.18 | 1.76±0.33 | 74.07±10.65†††,‡‡‡ |
| 13525 WT | 38.5±2.55 | 175.26±9.28 | 220.03±15.72 | 4.90±0.66 | 1.41±0.24 | 61.06±6.07 |
| 13525pYC | 234.3±10.86††† | 90.58±7.82††† | 233.43±17.55 | 90.58±3.42†† | 1.18±0.21 | 66.13±9.26 |
| 13525Int | 237.9±7.98††† | 31.80±5†††,‡‡‡ | 230.06±11.99 | 31.80±2.94† | 1.58±0.32 | 43.53±6.61‡ |
| P109 WT | 75.9±9 | 158.56±32.97 | 156.86±23.89 | 158.56±4.76 | 1.38±0.24 | 87.08±11.98 |
| P109pYC | 165.9±4.95††† | 117.96±15.36 | 180.46±13.53 | 117.96±2.59 | 2.08±0.34 | 26.24±8.26††† |
| P109 Int | 141.9±4.68†††,‡ | 126.02±9.42 | 191.83±12.87 | 126.02±5 | 1.73±0.26 | 37.51±5.6††† |
| Fp315 WT | 63.8±7.26 | 42.22±7.95 | 256.8±12.89 | 42.22±2.41 | 2.37±0.35 | 35.57±6.6 |
| Fp315pYC | 167.5±5.2††† | 23.18±4.92† | 263.92±15.98 | 23.18±5.14 | 2.07±0.18 | 73.84±9.09††† |
| Fp315 Int | 217.4±8.08††† | 27.37±4.69 | 245±22.63 | 27.37±4.4 | 2.77±0.49 | 79.16±8.39††† |
a
Activities are expressed as nmoles/min/mg total protein from mid-log to stationary phase cultures and are given as mean±SEM of readings from four independent observations.
†
Comparison of parameters with WT, ‡ comparison of parameters between pYC and Int. †††, ‡‡‡: P<0.001; ††, ‡‡: P<0.01; †, ‡: P<0.05.