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. 2014 Sep;6(9):1193–1199. doi: 10.3978/j.issn.2072-1439.2014.07.29

Table 1. Sequences of primers F3, B3, BIP and LB used in the real-amp assay.

Target Sequences(5’-’) of Strep-1 Sequences(5’-’) of Strep-2
F3 GGCTCTACTGTGAATTCTGG GGCTCTACTGTGAATTCTGG
B3 GGCAACTGGTACTGGTTC GGCAACTGGTACTGGTTC
FIP (F1c + F2) TATCCAGTCAGCGGACGGACTTGTCTGCCAGTGTTCC TATCCAGTCAGCGGACGGACTTGTCTGCCAGTGTTCC
BIP (B1c + B2) GCGCCTTCTTTAGCGTCTAAGTAACGAAGAAGGTGCCATG GCGCCTTCTTTAGCGTCTAAGTCAACGAAGAAGGTGCCAT
FLP ACAGGCTGGTACTACCTCA ACAGGCTGGTACTACCTCA
BLP GTACTTGACCCAGCCTGTC GTACTTGACCCAGCCTGTC

F3, forward outer primer; B3, backward outer primer; FIP, forward internal primer; B3, backward internal primer; FLB, forward loop backward primer; BLP, backward loop backward primer.