Figure 2. GJPs in Brn4-deficient cochleae are composed of Cx26 and Cx30 as in control littermates.

Inner sulcus cells in cochlear whole-mount sections from a 6-week-old littermate control (A–C) and Brn4-deficient (knockout, KO) mouse (D–F). Images were obtained by confocal microscopy and z-stacking after immunolabeling for Cx26 (green) (A, D) and Cx30 (red) (B, E). Merged images are also shown (C, F). Nuclei were counterstained with DAPI (blue) (A–F). Bars indicate 10 µm.