Figure 5. Gap junction protein levels were reduced in Brn4-deficient mice at 6 weeks.

Western blot analysis of Cx26 and Cx30 was performed in 6-week-old Brn4-deficient mice and littermate controls. The level of Cx26 and Cx30 was normalized to the corresponding β-actin levels and expressed relative to the amount present in each of the littermate controls. Values represent the mean ± SE (n = 3 mice). ***P = 7.943×10^–3 for Cx26 and ***P = 8.137×10^–3 for Cx30, Student’s t-test.