Figure 7. Fucci indicators reveal the exposure of human beta cells to a mitogen-rich medium.

Exponentially growing EndoC-βH2-PGF2AOF Fucci cells were seeded at either 80×10³ cells/cm² (A) or 40×10³ cells/cm² (B) in either standard medium or a mitogen-rich medium and analyzed for green and orange fluorescences through flow cytometry after 48 hours (left parts of the panels) or 96 hours (right parts of the panels). Doublets were excluded from the analysis. The percentages of cells displaying green, orange, «yellow» (double positive) or no fluorescence (« black », double negative) were plotted for each culture condition and time point.