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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Cancer Res. 2014 Jul 23;74(18):5277–5286. doi: 10.1158/0008-5472.CAN-14-0053

Figure 2.

Figure 2

Failed lobuloalveolar development in Runx2−/− mammary glands.

A) Whole mounts of transplanted Runx2+/+ and Runx2−/− mammary glands isolated from virgin Rag1−/− recipients at 16 weeks post-transplantation (Scale bar 1 mm).

B) H&E stained section through epithelial duct of glands shown in (A) (Scale bar 12.5 μm).

C-D) Same as in (A-B), but transplanted mammary glands collected at day 18.5 of pregnancy (P18.5) Arrows indicate lipid globules.

E) Sections of P18.5 Runx2+/+ and Runx2−/− mammary glands were analysed by immunofluorescence for polarity markers ZO1 (apical) and smooth muscle actin (SMA: basal).

F) Immunohistochemical analysis of β casein expression in Runx2+/+ and Runx2−/− mammary glands at P18.5 (Scale bar 25 μm). Higher magnification image shown in (G).