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(A) pERK, (B) total ERK, (C) pAKT, (D) total AKT (E) pGSKβ and (F) total GSKβ proteins quantified by western blot in response to repeated insulin stimulation. See Fig. 2 for measurement details. Data are represented as mean ± SD from three (170 nM) and four (17 nM) independent studies. Data normalization and symbols are as described in figure 1.
