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. 2014 Sep 26;9(9):e108770. doi: 10.1371/journal.pone.0108770

Figure 3. Analysis of RIG-I oligomerization in cellula as determined by co-immunoprecipitation 18 hours after stimulation by a cognate RNA ligand.

Figure 3

(A) Similar expression of Flag-RIG-I and cl25-RIG-I constructs in 293T cells as revealed by western blot. (B) Efficiency of Flag-RIG-I and Cl25-RIG-I to activate the IFN-β promoter after Poly(I:C) transfection. See figure 2 legend for details. (C, D) Lack of co-immunoprecipitation of Cl25-RIG-I with Flag-RIG-I after their co-transfection in Huh7.5 cells and stimulation with Poly(I:C), 5′pppssRNA(62-mer) or 5′pppdsRNA(62-mer) (C) or MeV infection (MOI 1) (D) as detected by western blot. (E) Nonsensical co-immunoprecipitation of Cl25-RIG-I with Flag-RIG-I expressed in 293T cells and after transfection of 1 µg or 50 ng of 5′pppds(or ss)RNA(62-mer) or MeV infection (MOI 0.5).