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. 2013 May 29;1:14. doi: 10.1186/2049-3002-1-14

Figure 1.

Figure 1

AS160_v2 is selectively upregulated in a subset of multiple myeloma cell lines and patient samples. (A) AS160 transcript levels in NBL and a panel of 8 MM cell lines assessed by qRT-PCR. Primers directed towards exon 1, common to full length and AS160_v2 transcripts were used in the quantitative RT-PCR analysis. Bars represent mean ± SD of three repeats; (B) Agarose gel electrophoresis of PCR products generated with primers flanking exons 11 and 12 from cDNA of MM.1S, KMS11, JJN3, and L363 cell lines. PCR product from a reaction using a vector containing full-length AS160 cDNA as template is included as a control; (C) Analysis of AS160 transcript abundance in the JJN3 cell line. AS160_v2 abundance in NBL (D), MM cell lines (E), and MM patient sample transcriptomes (F). Samples lacking AS160_v2 (12 of 82 normal B cells) were excluded.