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. 2013 May 29;1:14. doi: 10.1186/2049-3002-1-14

Figure 2.

Figure 2

AS160_v2 is phosphorylated in myeloma cells and introduction of a phospho-deficient AS160 mutant impairs constitutive GLUT4 plasma membrane localization. (A) Immunoblot analysis of AS160 protein levels in MM cell lines; (B) Immunoblot analysis of pThr642 AS160 protein levels in MM cell lines; (C) GLUT1 and GLUT4 localization in MM.1S cell line transiently co-transfected with GFP and either empty vector (pcDNA) or full-length AS160-4P; 48 h after transfection, GFP-positive cells were sorted and imaged for GLUT1 and GLUT4 via confocal immunofluorescence microscopy. Images and immunoblots are representative of three individual repeats.