Table 2.
Effects of FGFR4 deficiency on adipogenesis, adipose secretory function and metabolic pathways in breasts and breast tumor
| Proteins | WT | KO | Tg | KO-Tg |
|---|---|---|---|---|
|
1. Adipokines and adipose enzymes: |
|
|
|
|
| Adiponectin |
1 |
1.64 (0.23)* |
0.01 (0.002) |
0.11 (0.02) |
| Adipsin |
1 |
3.32 (0.17) |
0.12 (0.03) |
0.43 (0.06) |
| Leptin |
1 |
0.55 (0.04) |
0.003 (0.0001) |
0.05 (0.004) |
| Lipoprotein lipase |
1 |
0.92 (0.17) |
0.08 (0.02) |
0.25 (0.06) |
| Hormone sensitive lipase |
1 |
2.02 (0.31) |
0.11 (0.02) |
0.31 (0.03) |
|
2. PPAR gamma targets: |
|
|
|
|
| Fabp4 |
1 |
1.62 (0.26) |
0.04 (0.01) |
0.15 (0.03) |
| FASN |
1 |
1.01 (0.23) |
0.14 (0.03) |
0.35 (0.04) |
| IRS2 |
1 |
0.12 (0.03) |
1.84 (0.35) |
0.27 (0.08) |
| Glut4 |
1 |
0.66 (0.12) |
0.05 (0.003) |
0.18 (0.04) |
| SREBP1 |
1 |
0.27 (0.06) |
1.97 (0.29) |
0.33 (0.04) |
|
3. Adipogenesis regulation: |
|
|
|
|
| (1) Pro-adipogenesis |
|
|
|
|
| Cyclin D1 |
1 |
0.46 (0.10) |
6.61 (0.29) |
3.42 (0.18) |
| Cdk4 |
1 |
0.43 (0.08) |
5.14 (0.37) |
2.23 (0.19) |
| Cebpd |
1 |
0.24 (0.05) |
7.26 (0.48) |
2.46 (0.15) |
| E2F1 |
1 |
0.23 (0.03) |
2.16 (0.36) |
0.71 (0.11) |
| Jun |
1 |
0.56 (0.08) |
2.93 (0.26) |
1.52 (0.30) |
| Sfrp5 |
1 |
0.55 (0.10) |
0.01 (0.002) |
0.04 (0.005) |
| Src |
1 |
0.19 (0.03) |
4.72 (0.51) |
2.31 (0.38) |
| Wnt5b |
1 |
0.35 (0.06) |
3.01 (0.23) |
1.37 (0.29) |
| (2) Anti-adipogenesis |
|
|
|
|
| Cdkn1a (p21) |
1 |
1.65 (0.18) |
2.44 (0.14) |
3.71 (0.26) |
| Dlk1 |
1 |
0.31 (0.04) |
1.03 (0.13) |
0.12 (0.03) |
| Klf2 |
1 |
0.007 (0.001) |
1.23 (0.16) |
0.004 (0.0005) |
| (3) Others |
|
|
|
|
| Ppara |
1 |
7.33 (0.47) |
0.31 (0.05) |
1.36 (0.17) |
| Ucp1 | 1 | 177.1 (1.57) | 0.12 (0.02) | 9.94 (1.03) |
*mRNA levels were normalized to β-actin levels in the respective samples and then expressed as fold changes relative to that of the wild-type (WT) breasts, which is assigned as an arbitrary unit of 1. Data are mean (SD): P <0.05 (n = 4 for each group). FGFR, fibroblast growth factor receptor; KO, FGFR4 knockout mice; Tg, MMTV-TGFα transgenic mice; KO-Tg, FGFR4−/−: MMTV-TGFα bigenic mice.