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. 2014 Sep 29;4:266. doi: 10.3389/fonc.2014.00266

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Copyright © 2014 Wesa, Mandic, Taylor, Moschos, Kirkwood, Kwok, Finke and Storkus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometric analysis of antigen-specific CD4⁺ T cells isolated from melanoma patients. (A) Representative plots of flow cytometry analysis of HLA-DR*0401/peptide tetramer⁺CD4⁺ T cells from two melanoma patients (Mel046, Mel048). Live cells were gated based on forward and side scatter. Regions used to enumerate frequencies of (FluM1 or individual EphA2 or MAGE6 TAA) tetramer-reactive CD4⁺ T cells are shown. (B) Composite data obtained from flow cytometry analysis of CD4⁺ T cells using the indicated PE-labeled HLA-DR4/peptide tetramer probes in 15 HLA-DR*0401⁺ melanoma patients (Table 1; Mel031-Mel104). Box plots represent the range of responses for all patients, with mean frequencies shown as solid horizontal lines, and with whisker bars representing 5th and 95th percentiles for each HLA-DR4/peptide tetramer analyzed.