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. 2014 Sep 29;4:266. doi: 10.3389/fonc.2014.00266

Figure 6.

Figure 6

The Type-1 subset of TAA-specific CD4+ T cells is a minority cohort that exhibits preferential sensitivity to apoptosis in the peripheral blood of melanoma patients. Multi-parameter flow cytometry including intracellular staining for the Type-1 transcription factor T-bet (as described in Section “Materials and Methods”) was used to determine the frequency of Type-1 T cells among total CD4+ T cells or antigen-specific CD4+ T cell subsets [(A); n = 4; i.e., Mel612, Mel633, Mel634, Mel635], the frequency of apoptotic (Annexin-V+) events among Type-1 (T-bet+) vs. non-Type-1 TAA-specific CD4+ T cells (B,C). (B) Data derived from T cells obtained from patient Mel633.