Figure 10.
Knockdown of STC1 in the kidney is associated with greater macrophage infiltration. pTie2-Cre was delivered to the right kidneys of STC1 shRNA and scrambled shRNA Tg mice. Kidneys were harvested 4 days later, and methacarn-fixed sections were stained with rat anti-F4/80 (marker of macrophages/dendritic cells) or rabbit anti-CD3 (T-cell marker). Total F4/80+ and CD3+ cells infiltrating the cortex were counted, and the results were expressed as the total number of cells per 10 grids (1cm2 graded ocular grids viewed at ×20 magnification) spanning the entire cortex, using Nikon Eclipse 80i microscope system; bar graphs represents the mean±SEM of cell number from 10 grids. HPF, high-power field.