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. 2014 Apr 3;25(10):2303–2315. doi: 10.1681/ASN.2013070690

Figure 3.

Figure 3.

Expression of STC1 protein in proximal tubules and glomerular and peritubular capillaries: Loss of STC1 immunoreactivity in shRNA Tg kidneys after delivery of pTie2-Cre is accompanied by tubular vacuolization. pTie2-Cre was delivered to the right kidney of STC1 shRNA and scrambled shRNA Tg mice; kidneys were harvested 4 days later. Methacarn-fixed kidney sections were stained with rabbit anti-STC1 antibody (top panels); anti-CD31 (middle panels); periodic acid–Schiff (bottom panels). Sections from WT kidneys were included for comparison. Strong immunoreactivity for STC1 in peritubular and glomerular capillaries (arrows) and positive staining in epithelial cells (brown hue). Similar distribution of STC1 immunoreactivity is observed in WT kidneys and scrambled shRNA Tg kidneys that were treated with pTie2-Cre. Delivery of pTie2-Cre to STC1 shRNA Tg kidneys leads to knockdown of STC1 expression in peritubular and glomerular capillaries, as well as epithelial cells; the appearance of tubular vacuolization (arrowheads). Expression of the endothelial cell marker, CD31, is not affected by delivery of pTie2-Cre to STC1 shRNA or scrambled shRNA Tg kidneys.