Figure 1.

p53 is induced in ischemic and cisplatin nephrotoxic AKI in mice. Male C57BL/6 mice were (A–C) subjected to 28 minutes of bilateral renal ischemia followed by 0–2 days of reperfusion (n=20) or (D–F) injected with 30 mg/kg cisplatin (n=28) for 0–3 days of examination. In A, B, D, and E, blood samples were collected at indicated time points to measure BUN and serum creatinine. Data were expressed as means±SDs; the bars with different superscripts (a–c) in each panel were significantly different (P<0.05). In C and F, kidneys were harvested to extract cortical and outer medulla tissues at indicated time points for immunoblot analysis of p53 and cyclophilin B (loading control). I/R, ischemia/reperfusion.