Figure 3.

Ischemic and cisplatin-induced AKI is attenuated in PT-p53-KO mice. (A–D) Wild-type and PT-p53-KO littermate mice were subjected to 28 minutes of bilateral renal ischemia followed by 2 days of reperfusion or sham operation as control. (E–H) Wild-type and PT-p53-KO littermate mice were injected with 30 mg/kg cisplatin or saline as control for 3 days. (A, B, E, and F) Blood samples were collected for measurements of BUN and serum creatinine levels. Data were expressed as means±SDs (n=8); the bars with different superscripts (a–c) were significantly different from each other (P<0.05). In C and G, kidney cortical tissues were stained with hematoxylin-eosin to show histology. Original magnification, ×200. In D and H, tubular damage in I/R and cisplatin-treated cortical tissues was semiquantified as pathologic scores. Data were expressed as means±SDs (n=8). ^#P<0.05, significantly different from PT-p53-WT group. I/R, ischemia/reperfusion.