Figure 4.

AKI-associated renal apoptosis is suppressed in PT-p53-KO mice. Wild-type and PT-p53-KO littermate mice were subjected to 28 minutes of bilateral renal ischemia followed by 2 days of reperfusion or injected with 30 mg/kg cisplatin for 3 days. Control mice were sham-operated or injected with saline. Kidney cortical tissues were examined by TUNEL assay to reveal apoptosis. (A and B) Representative images of TUNEL staining. Original magnification, ×200. (C and D) Quantification of TUNEL-positive cells in the tissues from each condition. Data were expressed as means±SDs (n=8); ^#P<0.05, significantly different from the PT-p53-WT group. I/R, ischemia/reperfusion.