Ischemic AKI is diminished in PT-p53-KO mice, but not in OT-p53-KO mice. OT-p53-KO and wild-type littermate mice were subjected to 28 minutes of bilateral renal ischemia, whereas PT-p53-KO and wild-type littermate mice were subjected to 23 minutes of bilateral renal ischemia. Samples were collected after 2 days of reperfusion. (A) p53 expression in kidney tissues of OT-p53-KO and wild-type littermate mice. (B and C) BUN and serum creatinine, respectively. Data were expressed as means±SDs; bars with different superscripts (a–c) in each panel were significantly different (P<0.05). (D) Representative histology of cortical tissues from OT-p53-KO and wild-type littermate mice after hematoxylin-eosin staining. Original magnification, ×200. (E) Tubular damage score in renal cortical tissues from OT-p53-KO and wild-type littermate mice. I/R, ischemia/reperfusion.