Figure 7.

The induction of 31 genes during ischemic AKI is suppressed in PT-p53-KO mice. Wild-type (n=4) and PT-p53-KO (n=4) littermate mice were subjected to 28 minutes of bilateral renal ischemia followed by 24 or 48 hours of reperfusion or sham operation as control. (A) Total RNA samples isolated from renal cortical tissues were analyzed by a cDNA microarray containing 26,515 genes. The amount of the mRNA of each gene from the I/R group was divided by the amount of sham control to calculate the fold change. (B) The lysate of renal cortical tissues was examined by immunoblot analysis of Txnip, MLKL, DUSP5, SOCS2, and cyclophilin B. I/R, ischemia/reperfusion.