Figure 5.

SYK inhibition protects from the development of lung hemorrhage (LH) in experimental anti-GBM disease. (A) Lung injury was assessed by macroscopic inspection of the lung surfaces at the point of cull on day 36 and scored on the basis of the number of visible petechiae. Fostamatinib-treated animals were completely protected from developing macroscopic lung hemorrhage. B shows representative lung appearances for vehicle- and fostamatinib-treated animals. (C) Lung sections were also stained for hemosiderin with Perls’ Prussian blue (without counterstain), and the number of hemosiderin-positive cells were quantified using automated image analysis. Fostamatinib-treated animals had minimal evidence of hemosiderin deposition. (D) Photomicrographs show representative Perls’ stain in fostamatinib- and vehicle-treated groups. Original magnification, ×400. Animals: n=8/group. Data are reported as median per group±interquartile range. *P<0.05; **P<0.01.