Figure 3. N-terminal amino acid sequencing of the Asp49 phospholipase A2 N-terminal amino acid sequencing of the Asp49 phospholipase A2 peak shown in Fig. 1 (A; first ten residues), and snapshot of the fifth cycle chromatogram (B).

As expected for this type of enzyme, this cycle yields a strong signal corresponding to phenylalanine (Phe), while the retention time corresponding to leucine (Leu), a conserved position in Lys49 myotoxins, shows no traces of this amino acid residue.