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. 2014 Jun 12;35(10):2254–2263. doi: 10.1093/carcin/bgu133

Fig. 5.

Fig. 5.

Forced expression of PKCα impairs the inhibitory effect of miR-200b on cell migration and tumor metastasis. (A) Western blot analysis of PKCα, ZEB1 and E-cadherin levels in MDA-MB-231-GFP, MDA-MB-231-GFP-200b-pLenti6.3 and MDA-MB-231-GFP-200b-pLenti6.3-PKCα cells. (B) Wound healing cell migration assay for MDA-MB-231-GFP-200b-pLenti6.3 and MDA-MB-231-GFP-200b-pLenti6.3-PKCα cells. Scale bar = 100 µm. (C) Representative images of H&E staining of lung sections from mice with mammary fat pad injection of MDA-MB-231-GFP-200b-pLenti6.3 or MDA-MB-231-GFP-200b-pLenti6.3-PKCα cells. Scale bar = 100 µm. (D) Representative overlaid images of immunofluorescence staining of GFP (red color) with nuclear DAPI staining (blue color) in lung sections from mice with mammary fat pad injection of MDA-MB-231-GFP-200b-pLenti6.3 or MDA-MB-231-GFP-200b-pLenti6.3-PKCα cells. Scale bar = 50 µm. (E) Representative overlaid images of GFP direct fluorescence (green color) with nuclear DAPI staining (blue color) in lung sections from mice with mammary fat pad injection of MDA-MB-231-GFP-200b-pLenti6.3 or MDA-MB-231-GFP-200b-pLenti6.3-PKCα cells. Scale bar = 50 µm.

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