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C1q-depleted sera lack ficolin-2 but not related opsonins. Flow cytometry histograms show binding of ficolin-2 to serotype 11A pneumococci (A), MBL to Saccharomyces cerevisiae (B), and ficolin-1 to serotype 19C pneumococci (C) using 5% normal human sera (NHS) or C1q-depleted serum (C1q-dpl) from manufacturers A, B, and C.
