FIG 2.

Complement component-depleted sera are codepleted of ficolin-2. (A to C) Flow cytometry histograms show ficolin-2 binding to serotype 11A pneumococci in 5% C1q-depleted sera with medium control (A), rFicolin-2 supernatant (B), or C1q-depleted sera with rFicolin-2 supernatant (C). Shaded gray areas represent staining with secondary antibody only as a negative control. (D) Western blot for ficolin-2 in normal human sera and depleted sera. Lane 1, NHS; lane 2, C1q-depleted sera from manufacturer A (C1q A); lane 3, C1q-depleted sera from manufacturer B (C1q B); lane 4, C1q-depleted sera from manufacturer C (C1q C); lane 5, C4-depleted sera from manufacturer C (C4); lane 6, C3-depleted sera from manufacturer C (C3); lane 7, C2-depleted sera from manufacturer C (C2); lane 8, factor B-depleted sera from manufacturer C (fB).