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. 2014 Sep;21(9):1323–1329. doi: 10.1128/CVI.00370-14

FIG 3.

FIG 3

C1q-depleted sera can activate complement through MBL and therefore still contain MASPs. Shown are flow cytometry dot plots of C4 (x axis) and C3 (y axis) deposition on S. cerevisiae using 5% normal human serum (NHS) or C1q-depleted serum (C1q-dpl) from manufacturers A, B, and C. (A) Complement deposition using serum without inhibitor. (B) Complement deposition using serum with 100 mM d-mannose to inhibit MBL binding.