TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristics	Source or reference
E. coli strains
χ7213	thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4–2-Tc::Mu[λpir] ΔasdA4 Δ(zhf-2::Tn10); used for conjugational transfer of suicide plasmids	31
χ7232	endA1 hsdR17 (rK− mK+) glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR (φ80dlac Δ(lacZ)M15); used for general cloning	31
S. Gallinarum strains
χ4173	Wild-type challenge strain	7
287/91	Wild-type vaccine parent strain	29
χ11575	Δfur-453::cam	287/91
χ11386	ΔPrfaH178::TT araC PBAD rfaH	287/91; 7
χ11741	Δpmi-2426	287/91
χ11797	Δfur-712	287/91
χ11798	Δpmi-2426 Δfur-712	χ11741
χ11820	Δfur-712 Δpmi-2426	χ11797
χ11821	ΔPrfaH178::TT araC PBAD rfaH Δfur-712	χ11386
χ11822	ΔansB1235	287/91
χ11823	Δfur-712 ΔansB1235	χ11797
Plasmids
pKD46	λ red expression vector	30
pRE112	sacB mobRP4; R6K ori; Cmr	53
pYA3546	Suicide vector for introduction Δpmi-2426	25, 32
pYA5239	Suicide vector for introduction Δfur-712	pRE112
pYA5272	Suicide vector for introduction ΔansB1235	pRE112