TABLE 4.

Characteristics of L. monocytogenes isolates detected in soil samples between 2007 and 2008

Isolate code(s)	Region(s) and date(s) (mo, yr) of isolation	STa	PCR serogroups	AscIb	ApaIc	inlA typed	SSI-1e	actAf (268 bp)
ER 3, ER 5	B (9, 2007)	2	4b, 4d, 4e	SOM1, SOM2	SOM1, SOM2	2	−	−
ER 101	B (9, 2007)	4	4b, 4d, 4e	SOM3	SOM3	2	−	−
E 14	A (6, 2007)	1	4b, 4d, 4e	SOM4	SOM4	2	−	−
ER 99	B (9, 2007)	6	4b, 4d, 4e	SOM5	SOM5	2	−	+
ER 87, ER 96	B (9, 2007); B (12, 2007)	517	4b, 4d, 4e	SOM8	SOM8	2	+	+
ER 1, ER 13	B (9, 2007)	59	1/2b, 3b	SOM6, SOM7	SOM6, SOM7	2	−	+
E 34, E 35, E 38, ER 41, ER 58, ER 111	B (6, 2007); A, B (10, 2007); A (11, 2007)	37	1/2a, 3a	SOM9	SOM9	1	−	−
ER 29	A (9, 2007)	120	1/2a, 3a	SOM10	SOM10	3	+	−
ER 137	B (3, 2008)	7	1/2a, 3a	SOM11	SOM11	5	+	−
ER 75	B (11, 2007)	26	1/2a, 3a	SOM12	SOM12	4	+	−
ER 173	A (8, 2008)	177	1/2a, 3a	SOM13	SOM13	5	−	−
ER 54	B (10, 2007)	89	1/2a, 3a	SOM14	SOM14	1	−	+
ER 98	B (9, 2007)	21	1/2a, 3a	SOM15	SOM15	3	−	+
ER 132, ER 169	B (3, 2008); A (8, 2008)	91	1/2a, 3a	SOM16, SOM17	SOM16, SOM17	3	−	−
ER 21, ER 25, ER 67	A (9, 2007); A (11, 2007)	101	1/2a, 3a	SOM18	SOM18	6	−	+
ER 28	A (9, 2007)	20	1/2a, 3a	SOM19	SOM19	1	−	−

^aST, MLST sequence type.

^bAscI, PFGE type after restriction digest with the enzyme AscI.

^cApaI, PFGE type after restriction digestion with the enzyme ApaI.

^dinlA, PCR restriction fragment length polymorphism (RFLP) type for the detection of point mutations in the 733-bp fragment of the inlA gene; RFLP types 1 and 4 with truncation in inlA gene.

^ePresence or absence of SSI-1 (stress survival islet 1), in L. monocytogenes intergenic region lmo0443–lmo0449.

^fPolymorphism in the actA gene, producing a fragment of 268 bp instead of the expected 385 bp.