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Native PAGE shows that the Cry operon forms a complex. (A) The entire Cry operon (CryO) forms a complex of about 170 kDa in 24-h recombinant B. thuringiensis cultures. This complex included the Cbm17.1 protein as shown in the immunoblot (B). None of the other three constructs (pCry16, pCry16/17, and pCryO/Δ17.1) showed the presence of the complex in recombinant B. thuringiensis cultures.
