TABLE 2.
Primers used in this study
| Analysis and primer | Sequence (5′–3′)a | Descriptionb |
|---|---|---|
| Northern hybridization | ||
| 17N1F | AAAAAATCTAGAGAGTAAATCGGAAGAGATCC | Internal ORF23 |
| 17N1R | AAAAAAGATATCCTTATCCTTCTTCATGTTC | Internal ORF23 |
| 17N6F | AAAAAATCTAGAGCACTGAAGAAGAACGGTGC | Internal TMP |
| 17N6R | AAAAAAGATATCCCGCCCACGTTTGAATGTTG | Internal TMP |
| 17N6FN | AAAAAATCTAGACCAGCTGATTACTCCAGTCGG | Internal holin |
| 17N6RN | AAAAAAGATATCCTTCATCTCCATTGCTTACAAC | Internal holin |
| Primer extension | ||
| Ter1F | GTAGCTGAGCAAATGCAAAGTG | Amplifies Ter1 region |
| Ter1R | GCCTTTAAGTTTGATCGTCC | Amplifies Ter1 region |
| Ter2F | GGTATTCAAGCAATCAGCTTC | Amplifies Ter2 region |
| Ter2R | GAACTTGCTATTTCCGTTGGC | Amplifies Ter2 region |
| Ter3F | GAAGAGTTTGAGAGAATGCAAG | Amplifies Ter3 region |
| Ter3R | GTCTTGATTAACTCTGAAGAC | Amplifies Ter3 region |
| TERLF | GACGTTATACGAATGCACTACG | Within large terminase |
| TERLR | GTCGGGTATTTCGTACTAATGATC | Within large terminase |
| MHF | GAATGACTTGATTACTCGTGCG | Within major head |
| MHR | GCGTGATGGACTTGAACGTACC | Within major head |
| TMPF | GTCGTTGCGAGAAGTAAATTCCG | Within tail tape measure |
| TMPR | GCTTGCATTATCGGATTTGTCG | Within tail tape measure |
| GDGPF | GAAGACATCATCAAGTTCGCTCG | Within glycerophosphoryl |
| GDGDR | GCTCTTCTCCGTCTTTCATACC | Diester phosphodiesterase |
| ANTF | GTTACGAGTATTCGGAGCTAC | Within antireceptor |
| ANTR | CTGATCTCTCTCAAGTTCTTCTTG | Within antireceptor |
| RT-PCR | ||
| PE2 | AGCGACTGAAACTCCAAG | IRD800 primer for E1 transcript |
| PE5 | CTTGACCGACTGGAGTAATC | IRD800 primer for L1 transcript |
| PESeqE1F | GCAACTCATATATCAAAG | Amplifies TSS region for E1 |
| PESeqE1R | TGAGGATACAACTTGAAG | Amplifies TSS region for E1 |
| PESeqL1F | CCTAAGGCGATTGTATTTGAAGC | Amplifies TSS region for L1 |
| PESeqL1R | GGTAAGCGTGCACTTTCAATC | Amplifies TSS region for L1 |
a
Restriction sites are underlined.
b
TSS, transcription start site.