TABLE 2.
Analysis and primer | Sequence (5′–3′)a | Descriptionb |
---|---|---|
Northern hybridization | ||
17N1F | AAAAAATCTAGAGAGTAAATCGGAAGAGATCC | Internal ORF23 |
17N1R | AAAAAAGATATCCTTATCCTTCTTCATGTTC | Internal ORF23 |
17N6F | AAAAAATCTAGAGCACTGAAGAAGAACGGTGC | Internal TMP |
17N6R | AAAAAAGATATCCCGCCCACGTTTGAATGTTG | Internal TMP |
17N6FN | AAAAAATCTAGACCAGCTGATTACTCCAGTCGG | Internal holin |
17N6RN | AAAAAAGATATCCTTCATCTCCATTGCTTACAAC | Internal holin |
Primer extension | ||
Ter1F | GTAGCTGAGCAAATGCAAAGTG | Amplifies Ter1 region |
Ter1R | GCCTTTAAGTTTGATCGTCC | Amplifies Ter1 region |
Ter2F | GGTATTCAAGCAATCAGCTTC | Amplifies Ter2 region |
Ter2R | GAACTTGCTATTTCCGTTGGC | Amplifies Ter2 region |
Ter3F | GAAGAGTTTGAGAGAATGCAAG | Amplifies Ter3 region |
Ter3R | GTCTTGATTAACTCTGAAGAC | Amplifies Ter3 region |
TERLF | GACGTTATACGAATGCACTACG | Within large terminase |
TERLR | GTCGGGTATTTCGTACTAATGATC | Within large terminase |
MHF | GAATGACTTGATTACTCGTGCG | Within major head |
MHR | GCGTGATGGACTTGAACGTACC | Within major head |
TMPF | GTCGTTGCGAGAAGTAAATTCCG | Within tail tape measure |
TMPR | GCTTGCATTATCGGATTTGTCG | Within tail tape measure |
GDGPF | GAAGACATCATCAAGTTCGCTCG | Within glycerophosphoryl |
GDGDR | GCTCTTCTCCGTCTTTCATACC | Diester phosphodiesterase |
ANTF | GTTACGAGTATTCGGAGCTAC | Within antireceptor |
ANTR | CTGATCTCTCTCAAGTTCTTCTTG | Within antireceptor |
RT-PCR | ||
PE2 | AGCGACTGAAACTCCAAG | IRD800 primer for E1 transcript |
PE5 | CTTGACCGACTGGAGTAATC | IRD800 primer for L1 transcript |
PESeqE1F | GCAACTCATATATCAAAG | Amplifies TSS region for E1 |
PESeqE1R | TGAGGATACAACTTGAAG | Amplifies TSS region for E1 |
PESeqL1F | CCTAAGGCGATTGTATTTGAAGC | Amplifies TSS region for L1 |
PESeqL1R | GGTAAGCGTGCACTTTCAATC | Amplifies TSS region for L1 |
Restriction sites are underlined.
TSS, transcription start site.