TABLE 2.
Primers utilized for template cloning and qRT-PCR
| Primer name | Sequence (5′ to 3′) | Function (annealing temp [°C]) |
|---|---|---|
| 9766CLONINGF | CAAGTAAAAAAGGACACTGTTATGCG | Cloning spy coding region |
| 9766CLONINGR | CCGCTTTGTCATGCATCG | |
| 9766RTF2 | ATTATCGCCTCTGACAGCTTCGA | qRT-PCR (62) |
| 9766RTR2 | GGCGTTGGCGGTCATTTT | |
| 0526CLONINGF | TTTAATGTTATTTTTTCGAGGTTTTTGC | Cloning CKS-0458 coding region |
| 0526CLONINGR | AAACAGGTGACCGTCTTAC | |
| 0526RTFWD3 | CGGCACCAATTACGCGATCTAT | qRT-PCR (62) |
| 0526RTREV3 | GCCGGGATCACCGGATT | |
| YJBECLONING F | CCAATGTAGGTGTTGTTATGAAAAAACG | Cloning yjbE coding region |
| YJBECLONINGR | GTTGTGGTCGTGGTAGTTG | |
| YJBERTF | GCATTACTCGTCGCCGTCTCT | qRT-PCR (60) |
| YJBERTR | TGCTGCGGCACCAGCTT | |
| 8505CLONINGF | GAATGAGGGTAGACAAATGAAGG | Cloning CKS-0881 coding region |
| 8505CLONINGR | AGGATGTGATCAGAGGTGAG | |
| 8505RTF2 | GTGCAGAAGATGAATCTGACCAAAA | qRT-PCR (60) |
| 8505RTR2 | CATCCATTGGCGAAGTGGTTT | |
| CONIRTFWD | GAGCATCGTGGTGATTCAGGTAAT | Cloning CKS-1103 coding region |
| CONIRTREV | GCTGCTCTTTCCGCCTTTTTT | |
| 7180FWD2 | GCAGAGCATCGTGGTGATTCAG | qRT-PCR (60) |
| 7180REV2 | TGCTCTTTCCGCCTTTTTTACC | |
| 8895 CLONING F | CAAATGGCAAAATTAACCGTCCC | Cloning CKS-4689 coding region |
| 8895 CLONING R | TCAGAAGTTAAATCCGAGTTGC | |
| 8895RTF2 | CCCGGCGTTAATGACCAAA | qRT-PCR (64) |
| 8895RTR2 | CGCACTGGGAAAAATGATGCT | |
| FWDYCIFCLONING | ATGAGGCTCTTTTATGACTGTC | Cloning yciF coding region |
| REVYCIFCLONING | TCATTTCGCAACTCCTTCAG | |
| YCIFRTFWD | CCCTGGAAGGCCTGGTAGAA | qRT-PCR (60) |
| YCIFRTREV | GCGTACTTCACCCTTTTCAACAGA | |
| WCELCLONINGF | GGAATCACACCATGGCTCC | Cloning wceL coding region |
| WCELCLONINGR | CAACCAAAACTCCTGCGC | |
| WCELRTF | GGGTCGCTGGGTGAAAGG | qRT-PCR (62) |
| WCELRTR | CTGCCATCCATTGCGTAGGA | |
| LYSPCLONINGF | TCGCGCCTTATTTTTACCCAG | Cloning lysP coding region |
| LYSPCLONINGR | GCAGGCCAATATAGGTCGC | |
| LYSPRTF | GCCGTTTGCCGGAGGTT | qRT-PCR (64) |
| LYSPRTR | CCTGGAAAGAAAAGCCGACAA | |
| OSMYCLONINGF | GATGATATCGATGCACACAACTAAGC | Cloning osmY coding region |
| OSMYCLONINGR | TTTAACACTTTTTACGCCTTCGATAGC | |
| OSMYRTFWD | CGAAAATCGACAGCTCAATGAAGA | qRT-PCR (60) |
| OSMYRTREV | CCACCAGCGCCGCTTT | |
| WCEG2CLONINGF | CATTAAAGAGAGCAGGTTTTATGGC | Cloning wceG2 coding region |
| WCEG2CLONINGR | GGCAATATCCGTCCACAGC | |
| WCEG2RTF | GCGCGTCGCGAATGG | qRT-PCR (60) |
| WCEG2RTR | CCCCCGATGCGGGTAAT | |
| ELABCLONINGF | ATGTCAGGAAAAATTGAAGATGCCG | Cloning elaB coding region |
| ELABCLONINGR | TTACTTACGACCGAGCAGGAAG | |
| ELABRTF | CCCGCCGCTACACCAATC | qRT-PCR (62) |
| ELABRTR | CAAACGGGTTAGACTGCATCTGAT | |
| 27F | AGAGTTTGATCATGGCTCAG | Cloning 16S rRNA coding region |
| 1429RLONG | ACCTTGTTACGACTTCACC | |
| 16S-RTF | GCCAGCAGCCGCGGTAAT | qRT-PCR (60) |
| 16S-RTR | CGCTTTACGCCCAGTAATTCC |