TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant features | Reference or source |
|---|---|---|
| Strains | ||
| Rhizobium etli | ||
| CFN42 | Wild-type strain; Nalr | Laboratory collection |
| CFNX192 | CFNX89/p42d::Tn5-mob Nalr Kmr Neor | 18 |
| R. phaseoli | ||
| CIAT652 | Wild-type strain; Nalr | 43 |
| CCGM1 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| CCGM1.1 | CCGM1::Tn5-mob Nalr Kmr Neor | This study |
| CCGM2 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| CCGM8 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown with combined nitrogen; Nalr | This study |
| CCGM9 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown with combined nitrogen; Nalr | This study |
| R. leguminosarum | ||
| CCGM4 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| CCGM5 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| CCGM6 | Strain isolated from nodules of noninoculated P. vulgaris cv. Flor de Mayo plants grown in N-free medium; Nalr | This study |
| R. grahamii CCGM3 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| Agrobacterium tumefaciens | ||
| GMI9023 | C58 cured of its native plasmids | 56 |
| GMI9023/p42d | GMI9023/p42d::Tn5-mob Nalr Kmr Neor | 18 |
| S. americanum | ||
| CCGM7 | Strain isolated from nodules of noninoculated P. vulgaris cv. Negro Jamapa plants grown in N-free medium; Nalr | This study |
| CCGM7-dsRed | CCGM7 derivative with plasmid pFAJ1708::dsRed; Tcr | This study |
| Escherichia coli | ||
| HB101 | F− hsdS20-recA13 | 57 |
| S17-1 | recA1 thi pro Tra+ | 58 |
| Plasmids | ||
| pRK2013 | Helper plasmid; ColE1 mob+ Tra+ Kmr | 19 |
| pSUP5011 | Tn5-mob vector; Neor | 21 |
| pFAJ1708::dsRed | dsRed fluorescence gene from pHKT3, cloned on the SacI site of the pFAJ1708 vector; Tcr | J. Althabegoiti and G. Torres-Tejerizo, unpublished data |