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. 2014 Sep;80(18):5644–5654. doi: 10.1128/AEM.01491-14

TABLE 3.

Pyruvate dehydrogenase activity and poly-β-hydroxybutyrate accumulation in strains CCGM1 and CCGM7

Strain PDH activity (nmol NADH min−1 mg of protein−1)a,b PHB accumulation (mg PHB mg of protein−1)a,c
1st subculture 2nd subculture 3rd subculture
CCGM1 18.07 ± 1.15 0.072 ± 0.001 0.674 ± 0.12 3.464 ± 0.22
CCGM7 68.80 ± 1.43 0.468 ± 0.03 0.376 ± 0.05 0.463 ± 0.05
CIAT652 20.37 ± 0.20 0.061 ± 0.01 2.055 ± 0.03 4.161 ± 0.12
a

Values are averages ± standard deviations (n = 3).

b

The cells were grown for 8 h in minimal medium with 10 mM succinic acid and 10 mM ammonium chloride.

c

Measured at 24 h of growth of the 1st, 2nd, or 3rd subculture.