FIG 3.

Bacillaene inhibits M. xanthus predation. (A) Predation assays using the domesticated, ancestral, and mutant strains of B. subtilis. Mutations in sfp and pksL reveal the requirement for bacillaene as the major factor inhibiting M. xanthus predation. Pictures were taken 24 h after spotting M. xanthus predator cells in the center of the prey source. Bar, 0.1 cm. (B) Prey survival was quantified as described for Fig. 2 and normalized to that for the NCIB3610 control spotted without the predator. (C) Growth of the M. xanthus predator was quantified after 24 h and normalized to that for M. xanthus cells spotted without prey.