FIG 5.

Bacillus subtilis spores resist predation. Spores were made and purified as described in Materials and Methods (36). Spores were spotted on MOPS agar plates lacking any nutrients to prevent spore germination. Predation assays were conducted as shown in Fig. 1. Shown are spores alone (A), spores with predator cells (B), and vegetative prey cells with the predator (C). Domesticated strains as well as the NCIB3610 pksL mutant cells are capable of producing predation-resistant spores. Bar, 0.5 cm.