TABLE 1.

Lineage-specific detection of L. reuteri in wheat and sorghum sourdoughs propagated with 72-h and 12-h fermentation cycles^d

Fermentation time and sourdough mixture	Strain(s) of L. reuteri detected after indicated no. of fermentation cyclesc
	0	4	8	10	12
72 h
Wheat, cocktail 1a	HRS	RS	HRS	RS	RS
Wheat, cocktail 2b	HRS	HRS	HRS	RS	RS
Sorghum, cocktail 1	HRS	RS	RS	R	R
Sorghum, cocktail 2	HRS	R	R	R	R
12 h
Wheat, cocktail 1	HRS	HRS	HRS	HRS	HS
Wheat, cocktail 2	HRS	HRS	H	H	H
Sorghum, cocktail 1	HRS	HRS	HS	HS	HS
Sorghum, cocktail 2	HRS	H	H	H	H

^aCocktail 1 was composed of L. reuteri FUA3400 (human lineage II) (H), L. reuteri FUA5448 (rodent lineage I) (R), and L. reuteri FUA3168 (undefined lineage) (S).

^bCocktail 2 was composed of L. reuteri FUA3401 (human lineage II) (H), L. reuteri FUA5448 (rodent lineage I) (R), and L. reuteri FUA3324 (sorghum isolate, undefined lineage) (S).

^cStrains detected by observation of the lineage-specific melting peaks if they accounted for 1% or more of the total fermentation microbiota. Letters denote strains of the same host lineage.

^dDuplicate experiments were conducted with two strain cocktails. Lineage-specific detection of L. reuteri strains was achieved with HRM-qPCR.