TABLE 3.
Effect of oxidative stress modulators on thiol redox state in A. flavus
| Markerb | Stage | Value with no modulatora | Value with indicated ROS modulatora |
Value with indicated TRS modulatora |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Specific |
General |
||||||||||
| TIRON | DMSO | BHA | ATR | ASC | CAF | GSH | NAC | DTT | |||
| GSH | Control-SD | 6.5 ± 0.7 (1) | 51 ± 4.5 (7.9) | 7.7 ± 2 (1) | 6.8 ± 1.6 (1) | 38.7 ± 3.8 (6) | 9 ± 1.9 (1) | 5.15 ± 1.3 (1) | 20 ± 3.4 (3.1) | 5 ± 1.1 (1) | 11.1 ± 3.2 (1.7) |
| Control-UD | 6.4 ± 0.9 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-NSD | 7.7 ± 1.2 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-UD | 7.1 ± 0.8 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| GSSG | Control-SD | 16 ± 2.4 (1) | 92 ± 9.7 (5.8) | 34 ± 5 (2.1) | 25 ± 4.2 (1) | 44 ± 7.6 (2.8) | 37 ± 6.4 (2.3) | 22 ± 5 (1) | 145 ± 15 (9.1) | 38 ± 9.6 (2.4) | 17 ± 2.6 (1) |
| Control-UD | 18 ± 2.2 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-NSD | 27 ± 3.4 (1.7) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-UD | 20 ± 2 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| CSH | Control-SD | 2.6 ± 0.4 (1) | 4.3 ± 0.5 (1.7) | 4.5 ± 0.8 (1.7) | 4.2 ± 0.5 (1.6) | 3.7 ± 0.5 (1.4) | 10 ± 1.2 (3.9) | 2.5 ± 0.5 (1) | 5.9 ± 0.6 (2.3) | 13 ± 1.2 (5) | 15 ± 1.1 (5.8) |
| Control-UD | 2.1 ± 0.2 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-NSD | 2.5 ± 0.3 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-UD | 2.4 ± 0.4 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| CSHox | Control-SD | 37 ± 3 (1) | 22 ± 3 (0.6) | 41 ± 5 (1) | 46 ± 6 (1) | 54 ± 6 (1.5) | 40 ± 5 (1) | 32 ± 4 (1) | 121 ± 10 (3.3) | 81 ± 9 (2.2) | 37 ± 4 (1) |
| Control-UD | 39 ± 4 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-NSD | 48 ± 6 (1) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
| ΔveA-UD | 55 ± 5 (1.5) | NA | NA | NA | NA | NA | NA | NA | NA | NA | |
a
See footnote a of Table 1.
b
GSH (glutathione), GSSG (oxidized glutathione), CSH (cysteine), and CSHox (total oxidized CSH; the sum of values for cystine and CSH disulfides with other thiols) are measured in nmol/mg total fungal protein.