TABLE 3.
Amino acid sequence homology of introduced A domains aligned against the module 2 PvdD A domain
| Substitutiona | % identity | % similarity | % gaps |
|---|---|---|---|
| Thr-A | 99.6 | 99.8 | 0.0 |
| Ser-B | 50.6 | 64.1 | 7.3 |
| Lys-C | 52.3 | 64.9 | 5.9 |
| Thr-D | 64.7 | 77.4 | 2.3 |
| Thr-E | 65.1 | 77.3 | 3.5 |
| Ser-F | 47.6 | 61.5 | 5.7 |
| Asp-G | 40.7 | 56.3 | 5.3 |
| Gly-H | 47.1 | 62.3 | 6.8 |
| Ser-I | 46.8 | 61.7 | 6.9 |
| Thr-snbC m1 | 53.2 | 64.5 | 4.5 |
| Thr-syrB m9 | 44.1 | 60.2 | 5.4 |
| Cys-acvA m2 | 36.2 | 54.0 | 11.8 |
| Ser-pvdI m1 | 51.4 | 64.8 | 5.7 |
| Val-acvA m3 | 36.4 | 54.7 | 10.8 |
a
Substitutions created in this study are aligned with the A domain from Thr-WT. Substitutions from previous work (15) are in boldface. The nomenclature for A domain substitutions from the previous work shows the substrate activated by the module followed by the gene the module was sourced from and the number of the module within the named gene (15).