TABLE 1.
Oligonucleotide primers used in this study
| Primer | Oligonucleotide sequencea |
|---|---|
| sptC-F | 5′-CGGAATTCATATGACAAATAACAACGACCGAGACAG-3′ |
| sptC-R | 5′-TTTAAGCTTTCAGCAGTCCGCGACGTGTTCCCA-3′ |
| sptCH-R | 5′-TTTAAGCTTTCAGTGGTGGTGGTGGTGGTGGCAGTCCGCGACGTGTTCCCA-3′ |
| sptCΔC54H-R | 5′-TTTAAGCTTTCAGTGGTGGTGGTGGTGGTGCTCGCCGGTGCCGTCGTTGTC-3′ |
| sptCΔC143H-R | 5′-TTTAAGCTTTCAGTGGTGGTGGTGGTGGTGGTCGCCGCCCTCGTCGACGAG-3′ |
| sptCΔN127-F | 5′-GGGAATTCCATATGGAACAGCGCACCCCCGAC-3′ |
| sptCC151-F | 5′-GGGAATTCCATATGGCTCTCGTCGACGAGGGCGGC-3′ |
| sptCC54-F | 5′-GGGAATTCCATATGCAACCGGGCGACTGCGGCGAC-3′ |
| MChBD1 | 5′-GCTCTCGTCGACGAGGGCGGCCAACCGGGCGACTGCGGCGAC-3′ |
| MChBD2 | 5′-GTCGCCGCAGTCGCCCGGTTGGCCGCCCTCGTCGACGAGAGC-3′ |
| sptCS230A-F | 5′-GTCGGGCACGGCGATGGCGTCGC-3′ |
| sptCS230A-R | 5′-TCGCCGTGCCCGACAGCCGCTCGTAGCCGC-3′ |
a
Underlined sequences indicate the restriction enzyme sites used in this study. Bold indicates the mutated nucleotides. Italicized sections indicate the His6 tag-encoding DNA sequences.