TABLE 2.
Primer pairs, templates, and restriction enzyme sites used in plasmid construction
| Plasmid | Primer pair | Template | Restriction site |
|---|---|---|---|
| pET26b-sptC* | sptC-F + sptCH-R | Genomic DNA | NdeI-HindIII |
| pET26b-sptCΔCTE | sptC-F + sptCΔC143H-R | Genomic DNA | NdeI-HindIII |
| pET26b-sptCΔChBD | sptC-F + sptCΔC54H-R | Genomic DNA | NdeI-HindIII |
| pET26b-sptCΔPkdD | sptC-F + MChBD2 (5′ end of the gene) | Genomic DNA | |
| MChBD1 + sptCH-R (3′ end of the gene) | Genomic DNA | ||
| sptC-F + sptCH-R (3rd-round PCR) | 2nd-round PCR product | NdeI-HindIII | |
| pET26b-sptCΔN | sptCΔN127-F + sptCH-R | Genomic DNA | NdeI-HindIII |
| pET26b-CTE* | sptCC151-F + sptCH-R | Genomic DNA | NdeI-HindIII |
| pET26b-PkdD* | sptCC151-F + sptCΔC54H-R | Genomic DNA | NdeI-HindIII |
| pET28a-ChBD* | sptCC54-F + sptC-R | Genomic DNA | NdeI-HindIII |
| pET26b-sptCS230A | sptCS230A-F + sptCS230A-R | pET26b-sptC |