TABLE 2.

Primers used in this study

Virus, technique, and primer	Sequence	Gene product	Segment	Amplicon size (bp)	Reference
IPNV
RT-PCR
SolesegA-F2	5′-GTGCTGGCCACAAACGACAAC-3′	VP2	A	599	7
SolesegA-R2	5′-ATTTGGTCTGCCGTTCCTA-3′	VP2	A
Seminested RT-PCR
R23	5′-TCAACCAGCAGACAGCAATCGACAATG-3′	VP2	A	191	7
R24	5′-GTTTGGGATCAGCTCGTAGTTGGACAC-3′	VP2	A
RT-PCR
Heppell F	5′-AGAGATCACTGACTTCACAAGTGAC-3′	VP2	A	359	6
Heppell R	5′-TGTGCACCACAGGAAAGATGACTC-3′	VP2	A
Seminested RT-PCR, Heppell F intro	5′-AAAGGCATGGGGCTGGAGAG-3′	VP2	A	323	Dopazo et al., unpublished data
RT-PCR
D543F	5′-GAATCCMAACAAGACTCC-3′	VP1	B	462	Dopazo et al., unpublished data
D1005R	5′-GTAGGGTAGGCCGGCTGAGGACTT-3′	VP1	B
Seminested RT-PCR, N753R	5′-CACCATTGATAGYARTAGG-3′	VP1	B	210	Dopazo et al., unpublished data
VHSV
RT-PCR
cm3a	5′-CAGGCGTTGTCCGTGCTTCT-3′	N		358	12
cm3b	5′-ACCCTGCGGAGTTTCCTGATGG-3′	N
Nested RT-PCR
cm3a intro	5′-CTATGTACTCCAAGGGAAC-3′	N		310	12
cm3b intro	5′-CGGTGAAGTGCTGCAGTTC-3′	N
VNNV
RT-PCR
Noda-Fwr1	5′-CCTGARGASACCACCGCTCCMAT-3′	CP	RNA2	300	11
Noda-Rev2	5′-CSCCAWCTGTGAAYGTMTTGT-3′	CP	RNA2
RT-PCR
Noda F2	5′-CGTGTCAGTCATGTGTCGCT-3′	CP	RNA2	427	8
Noda R3	5′-AGTGTCTCCAGCTTTCTTCT-3′	CP	RNA2
Noda F2 ATL	5′-CGTGTCGGTGTTATGTCGCT-3′	CP	RNA2	427	47
Noda R3 ATL	5′-CGCATCGACCCTGGTGAAGG-3′	CP	RNA2
Nested PCR
Noda F2.2	5′-CRTCYCTYGAGACACCTGA-3′	CP	RNA2	179	Dopazo et al., unpublished data
Noda R3.2	5′-TGTARTCAATGGRCARCGG-3′	CP	RNA2
RT-PCR
Noda F7	5′-ATATCACGATGAGTTCACTA-3′	Protein A	RNA1	641	50
Noda R7	5′-CGATTCACTATTTTCAAGTC-3′	Protein A	RNA1