TABLE 5.
Prevalence of fish viruses in wild populations in the Gulf of Cadiz as determined by RT-PCR and confirmed by nested PCR and/or DBH
| Order, family, and species or parameter | Common name | Results of oceanographic campaign(s)a: |
|||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I |
II |
III |
All |
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| nb | Depth (m)c | Locationd | IPNV | VHSV | VNNV | n | Depth (m) | Location | IPNV | VHSV | VNNV | n | Depth (m) | Location | IPNV | VHSV | VNNV | n | IPNV | VHSV | VNNV | ||
| Perciformes | |||||||||||||||||||||||
| Sparidae | |||||||||||||||||||||||
| Boops boops | Bogue | 1 | 21 | I.7 | 0/1e | 0/0 | 0/0 | 5 | 51 | II.1 | 0/1 | 0/5 | 0/0 | 10 | 46 | III.2 | 0/2 | 0/6 | 0/0 | 16 | 0/4 | 0/11 | 0/0 |
| Pagellus bellottii | Red pandora | 0 | 5 | 28 | II.3 | 0/0 | 0/2 | 0/1 | 10 | 46 | III.2 | 0/4 | 0/5 | 2/2 | 15 | 0/4 | 0/7 | 2/3 | |||||
| Pagellus erythrinus | Common pandora | 2 | 30, 21 | I.2, I.7 | 0/1 | 0/0 | 0/0 | 5 | 51 | II.1 | 0/5†† | 0/4 | 0/0 | 10 | 46 | III.2 | 0/1 | 0/9 | 2/3 | 17 | 0/7 | 0/13 | 2/3 |
| Pagellus acarne | Axillary seabream | 1 | 21 | I.7 | 0/0 | 0/0 | 1/1* | 5 | 51 | II.1 | 4/4‡‡ | 0/5 | 1/2 | 10 | 46 | III.2 | 0/1 | 0/0 | 0/0 | 16 | 4/5 | 0/5 | 2/3 |
| Diplodus vulgaris | Common two-banded seabream | 2 | 30, 21 | I.2, I.7 | 1/2 | 0/0 | 1/1† | 5 | 51 | II.1 | 4/4§§ | 1/3 | 0/0 | 10 | 93 | III.2 | 0/1††† | 0/0 | 0/0 | 17 | 5/7 | 1/3 | 1/1 |
| Diplodus bellootii | Senegal seabream | 0 | 5 | 56 | II.4 | 3/5∥∥ | 0/4 | 0/0 | 12 | 26 | III.4 | 0/1 | 0/1 | 0/3 | 17 | 3/6 | 0/5 | 0/3 | |||||
| Sparus aurata | Gilthead seabream | 1 | 21 | I.7 | 0/1 | 0/0 | 0/1 | 0 | 0 | 1 | 0/1 | 0/0 | 0/1 | ||||||||||
| Lithognathus mormyrus | Sand steenbras | 1 | 21 | I.7 | 0/1 | 0/0 | 0/0 | 0 | 0 | 1 | 0/1 | 0/0 | 0/0 | ||||||||||
| Spondyliosoma cantharus | Black seabream | 1 | 61 | I.4 | 1/1 | 0/0 | 1/1‡ | 0 | 0 | 1 | 1/1 | 0/0 | 1/1 | ||||||||||
| Summary for family Sparidae | 9 | 2/6 (22.2/66.7)f | 0/0 (0/0) | 3/4 (33.3/44.4) | 30 | 11/19 (36.7/63.3) | 1/23 (3.3/76.7) | 1/3 (3.3/10) | 62 | 0/10 (0/16.1) | 0/21 (0/33.9) | 4/8 (6.5/12.9) | 101 | 13/35 (12.9/34.7) | 1/44 (1.0/43.6) | 8/15 (7.9/15.0) | |||||||
| Mullidae | |||||||||||||||||||||||
| Mullus barbatus | Red mullet | 1 | 30 | I.2 | 0/0 | 0/0 | 1/1§ | 0 | 0 | 1 | 0/0 | 0/0 | 1/1 | ||||||||||
| Mullus surmuletus | Surmullet | 1 | 30 | I.2 | 0/0 | 0/0 | 0/0 | 4 | 51 | II.1 | 0/1*** | 0/1 | 0/1 | 10 | 46 | III.2 | 0/0 | 0/4 | 0/3 | 15 | 0/1 | 0/5 | 0/4 |
| Summary for family Mullidae | 2 | 0/0 (0/0) | 0/0 (0/0) | 1/1 (50.0/50.0) | 4 | 0/1 (0/25.0) | 0/1 (0/25.0) | 0/1 (0/25.0) | 10 | 0/0 (0/0) | 0/4 (0/40.0) | 0/3 (0/30.0) | 16 | 0/1 (0/6.3) | 0/5 (0/31.3) | 1/5 (6.3/31.3) | |||||||
| Carangidae, Trachurus mediterraneus | Mediterranean horse mackerel | 1 | 28 | I.1 | 0/0 | 0/0 | 0/0 | 5 | 51 | II.1 | 0/1 | 0/2 | 0/2 | 10 | 93.5 | III.1 | 0/2 | 0/7 | 0/5 | 16 | 0/3 | 0/9 | 0/7 |
| Other Perciformes | |||||||||||||||||||||||
| Haemulidae | |||||||||||||||||||||||
| Pomadasys incisus | Bastard grunt | 1 | 28 | I.1 | 0/1 | 0/0 | 0/0 | 0 | 0 | 1 | 0/1 | 0/0 | 0/0 | ||||||||||
| Plectorhinchus mediterraneus | Rubberlip grunt | 1 | 44 | I.9 | 0/1 | 1/1 | 0/0 | 0 | 0 | 1 | 0/1 | 1/1 | 0/0 | ||||||||||
| Scianidae, Arginosomus regius | Meagre | 1 | 28 | I.1 | 0/1 | 0/0 | 0/1 | 0 | 0 | 1 | 0/1 | 0/0 | 0/1 | ||||||||||
| Scombridae, Scomber scombrus | Atlantic mackerel | 1 | 107 | I.6 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Sphiraenidae, Sphyraena sphyraena | Barracuda | 1 | 28 | I.1 | 0/1** | 0/0 | 0/1 | 0 | 0 | 1 | 0/1 | 0/0 | 0/1 | ||||||||||
| Summary for other Perciformes | 5 | 0/4 (0/80.0) | 1/1 (20.0/20.0) | 0/2 (0/40.0) | 0 | 0 | 5 | 0/4 (0/80.0) | 1/1 (20.0/20.0) | 0/2 (0/40.0) | |||||||||||||
| Summary for order Perciformes | 17 | 2/10 (11.8/58.8) | 1/1 (5.9/5.9) | 4/7 (23.5/41.2) | 39 | 11/21 (28.2/53.9) | 1/26 (2.6/66.7) | 1/6 2.6/15.4 | 82 | 0/12 (0.0/14.6) | 0/37 (0/45.1) | 4/16 (4.9/19.5) | 138 | 13/43 (9.4/31.2) | 2/64 (1.5/46.4) | 9/29 (6.5/21.0) | |||||||
| Families belonging to other orders | |||||||||||||||||||||||
| Gadiformes, Gadidae, Micromesistius poutasso | Blue whiting | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 5 | 48 | II.2 | 0/1 | 0/2 | 0/0 | 0 | 6 | 0/1 | 0/2 | 0/0 | |||||
| Gadiformes, Macrouridae, Caelorinchus caelorinchus | Hollowsnout grenadier | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Clupeidormes, Engraulidae, Engraulis encrasicolus | Anchovy | 0 | 5 | 56 | II.4 | 0/2 | 0/1 | 0/0 | 20 | 27 | III.3 | 0/0 | 0/10 | 0/4 | 25 | 0/2 | 0/11 | 0/4 | |||||
| Clupeiformes, Clupeidae, Alosa fallax | Twaite shad | 1 | 90 | I.5 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Mugiliformes, Mugilidae, Liza ramada | Thinlip Gray mullet | 1 | 28 | I.1 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Batrachoidiformes, Batrachoididae, Halobatrachus didactylus | Lusitanian toadfish | 1 | 21 | I.7 | 0/0 | 0/0 | 1/1¶ | 0 | 0 | 1 | 0/0 | 0/0 | 1/1 | ||||||||||
| Pleuronectiformes, Soleidae, Microchirus azevia | Sole | 1 | 48 | I.3 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Scorpaeniformes | |||||||||||||||||||||||
| Triglidae, Chelidonichthys lucerna | Tub gurnard | 1 | 90 | I.5 | 0/0 | 1/1 | 0/1∥ | 0 | 0 | 1 | 0/0 | 1/1 | 0/1 | ||||||||||
| Sebastidae, Helicolenus dactylopterus | Blackbelly rosefish | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Chimaeriformes, Chimaeridae, Chimaera monstrosa | Rabbit fish | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Carcharhiniformes, Scyliorhinidae, Galeus melastomus | Blackmouth catshark | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Squaliformes, Etmopteridae, Etmopterus spinax | Velvet belly | 1 | 665 | I.8 | 0/0 | 0/0 | 0/0 | 0 | 0 | 1 | 0/0 | 0/0 | 0/0 | ||||||||||
| Summary for other families | 11 | 0/0 (0/0) | 1/1 (9.1/9.1) | 1/2 (9.1/18.2) | 10 | 0/3 (0/30.0) | 0/3 (0/30.0) | 0/0 (0/0) | 20 | 0/0 (0/0) | 0/10 (0/50.0) | 0/4 (0/20.0) | 41 | 0/3 (0/7.3) | 1/14 (2.4/34.1) | 1/6 (2.4/14.6) | |||||||
| Total | 28 | 2/10 (7.1/35.7) | 2/2 (7.1/7.1) | 5/9 (17.9/32.1) | 49 | 11/24 (22.5/49.0) | 1/29 (2.0/59.2) | 1/6 (2.0/12.2) | 102 | 0/12 (0/11.8) | 0/47 (0/46.1) | 4/20 (3.9/19.6) | 179 | 13/46 (7.3/25.7) | 3/78 (1.7/43.6) | 10/35 (5.6/19.6) | |||||||
Oceanographic campaigns were performed during November 2010 (campaign I), March 2011 (campaign II), and October to December 2011 (campaign III).
Number of fish sampled for viral analysis.
Depth of the trawling transect. Depths and temperatures: 20 to 30 m, around 18°C; 40 to 50 m, around 16.5°C; 90 to 100 m, around 15°C, and ≥600 m, around 13°C.
Capture locations: I.1, 36.4250N, 6.3510W; I.2, 36.4750N, 6.3990W; I.3, 36.5640N, 6.5230W; I.4, 36.4310N, 6.4400W; I.5, 36.4270N, 6.4760W; I.6, 36.3800N, 6.4710W; I.7, 36.5600N, 6.4160W; I.8, 36.1960N, 6.0400W; I.9, 36.1820N, 6.2000W; II.1, 36.2320N, 6.2380W; II.2, 36.2020N, 6.5090W; II.3, 36.4750N, 6.4030W; II.4, 36.2760N, 6.2850W; III.1, 36.2260N, 6.3280W; III.2, 36.1820N, 6.2000W; III.3, 36.5780N, 6.4600W; III.4, 36.4250N, 6.3510W).
Number of positive fish, as determined by RT-PCR/nested PCR and/or DBH.
Prevalence percentage determined by RT-PCR/nested PCR and/or DBH. Code(s) of viral isolates obtained from certain fish species: *, SpPa-IAusc1551.10; †, SpDv-IAusc1549.10; ‡, SpSpc-IAusc1556.10; §, SpMb-IAusc1544.10; ¶, SpHd-IAusc1547.10; ∥, SpChl-IAusc1554.10; **, SpSps-IAusc1538.10; ††, SpPe-IAusc531.11 and SpPe-IAusc535.11; ‡‡, SpPa-IAusc526.11, SpPa-IAusc527.11, SpPa-IAusc528.11, and SpPa-IAusc530.11; §§, SpDv-IAusc518.11, SpDv-IAusc519.11, and SpDv-IAusc520.11; ∥∥, SpDb-IAusc525.11; ***, SpMs-IAusc541.11; †††, SpDv-IAusc221.12.