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Saturation of 125I-Vip3Aa binding to S. frugiperda BBMV. A fixed amount of BBMV (2 μg) was incubated with increasing amounts of 125I-Vip3Aa for 90 min. The reaction was stopped by centrifugation, and the radioactivity retained in the pellet was measured. Specific binding was determined by subtracting nonspecific binding from the total binding.
