TABLE 1.

Influence of pH, NaCl concentration, chelating agents, and the presence or absence of divalent cations on the binding of ¹²⁵I-Vip3Aa to S. frugiperda BBMV^a

Bufferb	Binding condition				125I-Vip3Aa binding (% of input)		Ratio of specific to total binding
	pH	NaCl concn (mM)	Divalent cation	EDTA concn (mM)	Specific	Total
A	9	150			0	6.3	0
	8.2				2.5	7.1	0.35
B	7.4	150			4.1	8.5	0.48
		0			1.9	5.8	0.33
		50			3.5	7.5	0.47
		100			3.8	8.5	0.45
		300			2.7	8.1	0.33
		500			1.8	7.5	0.24
		150		5	2.8	7.2	0.39
A	7.4	150	MnCl2 (10 mM)		0	21.8	0
			MnCl2 (1 mM)		5.5	13.1	0.42
			CuSO4 (1 mM)		0	8.2	0
			CuSO4 (0.1 mM)		2.5	8.2	0.30
			ZnCl2 (1 mM)		4.2	10.8	0.39
			MgCl2 (10 mM)		1.2	6.9	0.17
			CaCl2 (1 mM)		2.4	7.0	0.34

^aValues are the means of at least two replicates.

^bBuffer A is 20 mM Tris–0.1% BSA, and buffer B is 10 mM Na₂HPO₄/NaH₂PO₄-0.1% BSA.